Silver staining and recovery of AFLP amplification products on large denaturing polyacrylamide gels.
نویسندگان
چکیده
Thompson, L.A. Strasheim and R.R. Burgess. 1990. Purification and characterization of proximal sequence element-binding protein 1, a transcription activating protein related to Ku and TREF that binds the proximal sequence element of the human U1 promoter. J. Biol. Chem. 265:17911-17920. 20.May, G., C. Sutton and H. Gould. 1991. Purification and characterization of Ku-2, an octamer-binding protein related to the autoantigen Ku. J. Biol. Chem. 266:3052-3059. 21.McConnell, K. and W.S. Dynan. 1996. The DNA-dependent protein kinase: a matter of life and (cell) death. Curr. Opin. Cell Biol. 8:325-330. 22.Mimori, T., M. Akizuki, H. Yamagata, S. Inada, S. Yoshida and M. Homma. 1981. Characterization of a high molecular weight acidic nuclear protein recognized by autoantibodies in sera from patients with polymyositis-scleroderma overlap. J. Clin. Invest. 68:611-620. 23.Mitsis, P.G. and P.C. Wensink. 1989. Purification and properties of yolk protein factor I, a sequence-specific DNA-binding protein from Drosophila melanogaster. J. Biol. Chem. 264:5195-5202. 24.Paillard, S. and F. Strauss. 1991. Analysis of the mechanism of interaction of simian Ku protein with DNA. Nucleic Acids Res. 19:5619-5624. 25.Quinn, J.P. and A.R. Farina. 1991. Autoimmune antigen Ku is enriched on oligonucleotide columns distinct from those containing the octamer binding protein DNA consensus sequence. FEBS Lett. 286:225-228. 26.Roberts, M.R., W.K. Miskimins and F.H. Ruddle. 1989. Nuclear proteins TREF1 and TREF2 bind to the transcriptional control element of the transferrin receptor gene and appear to be associated as a heterodimer. Cell Regul. 1:151-164. 27.Shi, Y., E. Seto, L.-S. Chang and T. Shenk. 1991. Transcriptional repression by YY1, a human GLI-Krüppel-related protein, and relief of repression by adenovirus E1A protein. Cell 67:377-388. 28.Waldschmidt, R., I. Wanandi and K.H. Seifart. 1991. Identification of transcription factors required for the expression of mammalian U6 genes in vitro. EMBO J. 10:2595-2603. 29.Wang, J., M. Satoh, A. Pierani, J. Schmitt, C.H. Chou, H.G. Stunnenberg, R.G. Roeder and W.H. Reeves. 1994. Assembly and DNA binding of recombinant Ku (p70/p80) autoantigen defined by a novel monoclonal antibody specific for p70/p80 heterodimers. J. Cell Sci. 107:3223-3233. 30.Yoon, J.-B., S. Murphy, L. Bai, Z. Wang and R.G. Roeder. 1995. Proximal sequence element-binding transcription factor (PTF) is a multisubunit complex required for transcription of both RNA polymerase IIand RNA polymerase III-dependent small nuclear RNA genes. Mol. Cell. Biol. 15:2019-2027.
منابع مشابه
A silver staining procedure for nucleic acids in polyacrylamide gels without fixation and pretreatment.
Silver staining of nucleic acid has been widely used in molecular marker analysis such as simple sequence repeat (SSR), single-strand conformation polymorphism (SSCP), and amplified fragment-length polymorphism (AFLP). Many alternatives to silver staining methods have been described, but these methods are not efficient or cost-effective. Here we report a silver staining method that requires les...
متن کاملPolymorphism of LPL Locus in Japanese and comparison of PCR amplification efficiency from degraded DNA between LPL locus and the D21S11.
The short tandem repeat (STR) polymorphism of the lipoprotein lipase (LPL) locus was amplified by PCR and analyzed using denaturing polyacrylamide gel electrophoresis followed by silver staining. Among 158 DNA samples from the Japanese population, six alleles were observed. When the sequences of the allelic products were compared, each allelic segment contained 7 and 9-13 TTTA tetranucleotide r...
متن کاملGeneral approach to analysis of polymorphic short tandem repeat loci.
Polymerase chain reaction amplification products of 22 known polymorphic short tandem repeat (STR) loci were subjected to denaturing polyacrylamide gel electrophoresis and detected using a silver staining method. Loci that amplified efficiently and revealed the fewest amplification-related artifacts with this detection method were selected for development of allelic ladders. The combination of ...
متن کاملDDRT-PCR: use of agarose gels for detection of amplified products.
The present study focuses on the detection of differentially expressed genes in migrating (healing) and nonmigrating (normal) corneal epithelium on agarose gel using a modified procedure of differential display reverse transcriptase-polymerase chain reaction (DDRT-PCR). Rabbit corneal epithelial organ cultures were used to obtain nonmigrating and migrating samples. RNA was extracted using Trizo...
متن کاملSilver and Cyanine Staining of Oligonucleotides in Polyacrylamide Gel.
To explore why some oligonucleotides in denaturing polyacrylamide gel could not be silver-stained, 134 different oligonucleotides were analyzed using denaturing polyacrylamide gel electrophoresis stained with silver and asymmetric cyanine. As a result, we found that the sensitivity of oligos (dA), (dC), (dG) and (dT) to silver staining could be ranged as (dA) > (dG) > (dC) > (dT) from high to l...
متن کاملThe DYS19 system in the Japanese population and its detection using teeth as a source of DNA.
The Y-specific short tandem repeat (STR) polymorphism of the locus DYS19 was amplified by PCR and analyzed by denaturing polyacrylamide gel electrophoresis followed by silver staining. Among 119 DNA samples from Japanese males, five alleles were observed. When sequences of the products were compared, each allelic segment contained 13 to 17 GATA tetranucleotide repeats, and revealed no differenc...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- BioTechniques
دوره 22 2 شماره
صفحات -
تاریخ انتشار 1997